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KMID : 1094720230280040672
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Biotechnology and Bioprocess Engineering
2023 Volume.28 No. 4 p.672 ~ p.683
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Bioimprinted Aspergillus niger Lipase Combined with Adsorption Immobilization by Resin and Its Experiment in Catalytic Synthesis of Sucrose-6-acetate
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Junqing Qian
Andrew H. Shitta
Jeneral D. Alfin
Changyan Zhao
Yiao Tang
Hui Guo
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Abstract
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The technology of bioimprinting combined with adsorption immobilization by resin of Aspergillus niger lipase was studied for enhancing the catalytic esterification performance detected by model reaction (enzymatic synthesis of sucrose-6-acetate) of lipase. The immobilization yield of lipase on resin was 95.7 ± 1.9% after optimized adsorption conditions. Under the optimal reaction conditions, the esterification conversion and 6-hydroxy esterification conversion of model reaction catalyzed by immobilized lipase were 72.6 ± 0.4% and 83.6 ± 0.3%. The bioimprinting of A. Niger lipase was studied and the imprinting conditions were optimized based on the immobilization technique. When imprinted immobilized lipase was applied to the model reaction, the esterification conversion and 6-hydroxy esterification conversion were reached 82.4 ± 0.1% and 82.5 ± 2.5%, respectively. The sucrose-6-acetate yield was 73.31 ± 2.2% by internal standard method. The results indicate that based on enzyme immobilization combined with bioimprinting technology could improve the esterification effect with basically unchanged esterification selectivity.
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KEYWORD
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bioimprinting, adsorption immobilization, catalytic esterification, lipase
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